Composite

Part:BBa_K227008:Design

Designed by: WashU iGEM 2009   Group: iGEM09_Wash_U   (2009-10-17)

ompC Promoter+pucBA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no evidence of ompC region having a natural binding promotor in R. sphaeroides, so this part requires an OmpR coding region elsewhere to function. As a synthesized part, this is not a true composite of BBa_R0082 (ompC) and BBa_K227006 (pucBA), but also includes ~40 bases upstream of the pucBA transcription start site, which includes the putative RBS region of R. sphaeroides.

Source

existing registry parts

References